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Limbal Stem Cell Transplant
Fig 1. Restoration of a healthy ocular surface by transplanting limbal stem cell construct to patient's affected eye.
Trypan blue staining
Fig 2. Trypan blue stain shows where acellular basement membrane remains after 7 days of limbal stem cell expansion over DM. Hyperfluorescent areas of Calcein AM demonstrates where living cells are. These cells express limbal stem cell markers p63a (purple nuclei) and ABCG2 (green cytoplasm), including groups of co-expressing cells (arrows).